CENTROSOMES IN DEVELOPMENT & DISEASE
Centrosomes are the microtubule organizing centers of most eukaryotic cells tasked with building the bipolar mitotic spindle that segregates the duplicated genome into two daughter cells during cell division. As such, centrosomes are integral to maintaining genome stability. Centrosomes orchestrate numerous critical tasks beyond cell division: cell polarization and migration, intracellular trafficking, and ciliogenesis.
Given these critical roles, centrosome dysfunction is the leading cause of microcephaly and is also associated with sterility, ciliopathy, poor cancer prognosis, and other disorders.
Cell cycle-dependent oscillations in composition and organization contribute to the multifunctional nature of centrosomes. We study how these dynamic changes instruct centrosome activity and function. Our goal is to elucidate mechanisms of centrosome regulation in the context of normal development and how these regulatory paradigms go awry in models of human disease or developmental disorders.
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Benner, L., Castro, E.A., Whitworth, C., Venken, K.J.T., Yang, H., Fang, J., Oliver, B.#, Cook, K.R., and Lerit, D.A.# (2019) Drosophila heterochromatin stabilization requires the zinc-finger protein Small Ovary. Genetics. (in press) # corresponding authors
Ryder, P.V. and Lerit, D.A. (2018) RNA localization regulates diverse and dynamic cellular responses. Traffic. 19(7):496-502.
Lerit, D.A.*#, Shebelut, C.W., Lawlor, K., Rusan, N.M., Gavis, E.R., Schedl, P., and Deshpande, G.*# (2017). Germ cell-less promotes centrosome segregation to induce germ cell formation. Cell Reports. 18(4): 831-839. * equal contributors, # corresponding authors
Lerit, D.A.*# and Poulton, J.S.*# (2016). Regulation of proper centriole number is critical for genomic stability. Chromosome Research. 24: 5-17. * equal contributors, # corresponding authors
Lerit, D.A., Jordan, H.A., Poulton, J.S., Fagerstrom, C.J., Galletta, B.J., Peifer, M., and Rusan, N.M. (2015) Interphase centrosome organization by the PLP-Cnn scaffold is required for centrosome function. Journal of Cell Biology. 210(1): 79-97.
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Galletta, B.J.*, Guillen, R.X.*, Fagerstrom, C.J., Brownlee, C.W., Lerit, D.A., Megraw, T., Rogers, G.C., and Rusan, N.M. (2014). Drosophila Pericentrin requires Calmodulin for its function in vivo. Molecular Biology of the Cell. 25(18): 2682-2694 * equal contributors
Lerit, D.A., Plevock, K.M., and Rusan. N.M. (2014). Live imaging of Drosophila larval neuroblasts. Journal of Visualized Experiments. (89), e51756.
Lerit, D.A. and Rusan, N.M. (2013). PLP inhibits the activity of interphase centrosomes to ensure their proper segregation in stem cells. Journal of Cell Biology. 202(7):1013-1022.
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Lerit, D.A., Smyth, J.T., and Rusan, N.M. (2013). Organelle asymmetry for proper fitness, function and fate. Chromosome Research. 21(3): 271-86.
Gopalakrishnan, J., Chim, Y.-C.F.*, Ha, A.*, Basiri, M.L.*, Lerit, D.A., Rusan, N.M., and Avidor-Reiss, T. (2012). Tubulin nucleotide status controls Sas-4-dependent pericentriolar material recruitment. Nature Cell Biology. 14(8): 865-873. * equal contributors
Becalska, A.N.*, Kim, Y.R.*, Belletier, N.G., Lerit, D.A., Sinsimer, K.S., and Gavis, E.R. (2010). Aubergine is a component of a nanos mRNA localization complex. Developmental Biology. 349(1): 46-52. * equal contributors
Korpal, M., Yan, J., Lu, X., Xu, S., Lerit, D.A., and Kang, Y. (2009). Imaging transforming growth factor-b signaling dynamics and therapeutic response in breast cancer bone metastasis. Nature Medicine. 15(8): 960-966.
Department of Cell Biology
Emory University School of Medicine
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